rabbit polyclonal anti-e. coli clpb antibodies  (Delphi Genetics)

Journal: Microorganisms

Article Title: Host Starvation and Female Sex Influence Enterobacterial ClpB Production: A Possible Link to the Etiology of Eating Disorders

doi: 10.3390/microorganisms8040530

Figure Lengend Snippet: Effects of food restriction on ClpB production. ( A ) Fecal ClpB DNA levels before (solid circles) and 14 days after (empty circles) food restriction in male and female rats. ( B ) Biotyper identification of Enterobacteriaceae in fecal microbiota before (Bef.) and after (Aft.) restriction (R.) in female and male rats. ( C) ClpB protein concentration in the intestinal mucosa at the levels of the duodenum (Duo), jejunum (Jej), ileum (Ile), and colon (Col) in male (solid symbols) and female (empty symbols) rats after food restriction. ( D ) ClpB protein plasma concentration before (solid circles) and 14 days after (empty circles) food restriction in male and female rats. ( E ) Correlations between fecal ClpB DNA and plasma ClpB after food restriction in male (solid circles) and female (empty circles) rats. ( F ) Plasma levels of ClpB protein in male mice with activity-based anorexia (ABA) as compared to ad libitum-fed controls (CT) and feeding time-restricted (FTR) mice, plasma samples available from a previous study . ( A ) 2-way ANOVA, time interaction * p = 0.02. ( C ) Students t-test * p < 0.05, ** p < 0.01. ( D ) 2-way ANOVA, time (** p = 0.001) and row Factor (* p = 0.04). ( F ) 1-way ANOVA, Bonferroni’s post-test * p < 0.05, ** p < 0.01.

Article Snippet: For this purpose, 3 antibodies were used: Rabbit polyclonal anti- E. coli ClpB antibodies (Delphi Genetics, Gosselies, Belgium) as a capture antibody, mouse monoclonal anti- E. coli ClpB antibodies as a detection antibody (Delphi Genetics), and an alkaline phosphatase-conjugated goat anti-mouse revelation antibody (Jackson ImmunoResearch, Cambridgeshire, UK).

Techniques: Protein Concentration, Clinical Proteomics, Concentration Assay, Activity Assay

Journal: Microorganisms

Article Title: Host Starvation and Female Sex Influence Enterobacterial ClpB Production: A Possible Link to the Etiology of Eating Disorders

doi: 10.3390/microorganisms8040530

Figure Lengend Snippet: Effects of food restriction on ClpB- and α-MSH-reactive immunoglobulin production. ClpB- ( A , B , E , F ) and α-MSH-( C , D , G , H ) reactive free and total IgG ( A – D ) and IgM ( E – H ) before (CT) and after 14 days of feeding time restriction (FTR) in male (light and dark blue, left side of each panel) and female (light and dark pink, right side of each panel. A – D , F , H , Students t-test * p < 0.05, ** p < 0.01, *** p < 0.001, 1-way ANOVA, Bonferroni’s post-test # p < 0.05, ## p < 0.01.

Article Snippet: For this purpose, 3 antibodies were used: Rabbit polyclonal anti- E. coli ClpB antibodies (Delphi Genetics, Gosselies, Belgium) as a capture antibody, mouse monoclonal anti- E. coli ClpB antibodies as a detection antibody (Delphi Genetics), and an alkaline phosphatase-conjugated goat anti-mouse revelation antibody (Jackson ImmunoResearch, Cambridgeshire, UK).

Techniques:

Journal: Microorganisms

Article Title: Host Starvation and Female Sex Influence Enterobacterial ClpB Production: A Possible Link to the Etiology of Eating Disorders

doi: 10.3390/microorganisms8040530

Figure Lengend Snippet: Effects of sex hormones on in vitro ClpB production. Changes of ClpB levels vs. control (CT) after acute application of several doses of testosterone ( A ) or estradiol ( B ) on E. coli cultures. ( C ) Immunocytochemical detection (green fluorescence) of ClpB in E. coli cultures treated with 4 doses of estradiol and in non-treated control (CT) bacteria as well as in ClpB-negative (Delta-) and -positive (Delta +) E. coli mutant strains, DAPI counterstaining. Magnification x 100 ( D ) Percentage of changes of ClpB-positive vs. total bacteria after estradiol treatment as compared to non-treated control (CT) bacteria and ( E ) their correlations with ClpB concentrations in culture medium. Effects of chronic application of estradiol on E. coli cultures on ClpB concentrations in bacteria ( F ) and culture medium ( G ) as well as on the ClpB secretion rate ( H ) and ClpB mRNA levels ( I ). A , B , D , 1-way ANOVA, Bonferroni’s post-test * p < 0.05, ** p < 0.01, F , G , H , Students t-test * p < 0.05, ** p < 0.01.

Article Snippet: For this purpose, 3 antibodies were used: Rabbit polyclonal anti- E. coli ClpB antibodies (Delphi Genetics, Gosselies, Belgium) as a capture antibody, mouse monoclonal anti- E. coli ClpB antibodies as a detection antibody (Delphi Genetics), and an alkaline phosphatase-conjugated goat anti-mouse revelation antibody (Jackson ImmunoResearch, Cambridgeshire, UK).

Techniques: In Vitro, Control, Fluorescence, Bacteria, Mutagenesis